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Class Number: 935 Class Title: GENETIC ENGINEERING: RECOMBINANT DNA TECHNOLOGY, HYBRID OR FUSED CELL TECHNOLOGY, AND RELATED MANIPULATIONS OF NUCLEIC ACIDS Subclass Subclass Number Title 1 OBTAINING THE DESIRED GENE; DNA, RNA PER SE AND THE MODIFICATION THEREOF OTHER THAN VECTOR MODIFICATION 2 .DNA - RNA hybrid 3 .RNA 4 ..mRNA 5 ..2-100 nucleotides in length, e.g., t-RNA, etc. 6 .DNA, e.g., regulatory sequences, etc. 7 ..Homopolymeric, e.g., poly d(A) sequence, etc. 8 ..12-75 nucleotides in length, e.g., primers, etc. 9 ..Structural gene sequence 10 ...Modified structural gene, e.g., nonnaturally occuring sequence, etc. 11 ...Polypeptide 12 ....Antigenic material 13 ....Hormone, e.g., human growth factor, insulin, etc. 14 ....Enzyme 15 ....Antibody 16 .Methods of producing DNA or RNA other than by expression vectors, e.g., culture of cells high in DNA, etc. 17 ..Cell free production 18 ...cDNA synthesis 19 .Isolation or purification of DNA or RNA 20 ..RNA 21 ...mRNA 22 VECTORS AND METHODS OF MODIFYING VECTORS 23 .Inserting a gene into a vector to form a recombinant vector, i.e., cleavage and ligation 24 ..Vector utilized, e.g., episomes, etc. 25 ...Plant virus 26 ...Cosmid 27 ...Plasmid 28 ....Yeast 29 ....Prokaryotic 30 ....Plant 31 ...Bacteriophage 32 ...Animal virus, e.g., SV40, etc. 33 METHODS OF ENHANCING OR DIMINISHING EXPRESSION 34 .Eukaryotic cell 35 ..Plant cell 36 ..Transcription 37 ..Yeast cell 38 .Prokaryotic cell 39 ..Transcription 40 ...Operon selection 41 ....Promoter, e.g., portable promoters, etc. 42 ..Gene dosage modification, e.g., copy number amplification, etc. 43 ...Inducible, e.g., temperature inducible, etc 44 ..Translation 45 ...Ribosome binding sitee 46 ...Initiation 47 .Fused protein or peptide 48 ..Signal peptide, e.g., secretion, etc. 49 .Post translational modification 50 ..Glycosylation 51 ..Peptide bond cleavage 52 METHODS OF INTRODUCING A GENE INTO A HOST CELL, E.G., TRANSFORMATION OR TRANSFECTION, ETC. 53 .Microinjection 54 .Microencapsulation, e.g., liposome vesicle etc. 55 .Using vector, e.g., plasmid, etc. 56 ..Plasmid 57 ..Virus 58 ...Phage, e.g., phage lambda, etc. 59 METHOD OF USE OF GENETICALLY ENGINEERED CELLS, E.G., OIL SPILL CLEANUP, ETC. 60 .To produce an identified chemical product e.g., amino acid, etc. 61 ..Yield optimization 62 .Control of genetic diseases or defects by use of added gene 63 .Use im animal husbandry 64 .Use in agriculature 65 .Vaccine production 66 CELLS CONTAINING A VECTOR AND/OR EXOGENOUS GENE PER SE; PROPAGATION THEREOF; OTHER MEMBRANE ENCAPSULATED DNA, E.G., PROTOPLASTS, ETC. 67 .Plant cells 68 .Fungal cells 69 ..Yeast cells 70 .Animal cell 71 ..Human cell 72 .Bacteria 73 ..Escherichia 74 ..Bacillus 75 ..Streptomyces 76 ASSAY RELATED TO GENETIC ENGINEERING 77 .Methods of analysis of nucleic acids 78 ..Including hybridization 79 .Methods of selection of recombinant gene containing vector; materials therefore, e.g., replica plating, etc. 80 ..Gene library manipulation 81 ..Antigen-antibody 82 ..Enzyme activity 83 ..Host suicide 84 ..Selection medium 85 GENETIC ENGINEERING APPARATUS 86 .Analytical, e.g., for autoradiography, etc. 87 ..Automated 88 .Synthesis, e.g., peptide or gene synthesizers, etc. 89 HYBRID OR FUSED CELL TECHNOLOGY, E.G., HYBRIDOMA, ETC. 90 .Method of selection of the desired cell 91 ..Of plant cells, e.g., protoplasts, etc. 92 ..Using positive selection technique 93 .Method of production of hybrid or fused cells, e.g., chromosome or genome transfer techniques, etc. 94 ..Of plant cells 95 .Fused or hybrid cell per se 96 ..Interspecies fusionn 97 ..Fungi, e.g., yeasts, etc. 98 ..Plant cells 99 ..Human cell 100 ...B lymphocyte 101 ...T lymphocyte 102 ..Animal cell 103 ...Murine cell, e.g., mouse cell, etc. 104 ....B lymphocyte 105 ....T lymphocyte 106 .Method of use of the fused or hybrid cell or the product thereof 107 ..In vivo use of product 108 ..In vitro, e.g., cell cultivation techniques, affinity chromatography, etc. 109 ...Production of non-antibody product 110 ...For use as testing material 111 MISCELLANEOUS